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You are here:Home » Biochemicals » Biochem-Diagnostics » WST-1 (Monosodium salt, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium)

WST-1 (Monosodium salt,
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium)

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Specifications

A water-soluble tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 438nm. Useful in cell proliferation assays as a cell viability indicator.
Catalog #170101
FormulaC19H11IN5NaO8-S2
SolubilityH2O (~10mg/ml)
Molecular Weight~651.35
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number150849-52-8
Molecular FormulaC19H11IN5NaO8-S2
Molecular Weight~651.35
Purity~97% (HPLC)
ConcentrationNot determined
FormSupplied as a pale brown solid.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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