CA2 is a cytosolic member of the alpha class. It is the most widely distributed isoform among the mammalian CAs (1). Defects in CA2 are associated with osteopetrosis and renal tubular acidosis (3-5). Elevated expression of CA2 is observed in patients with Alzheimer’s disease and the developing brains of Down syndrome patients (6,7). CA2 is also overexpressed in Gastrointestinal Stromal Tumors (GISTs) and is considered a useful marker for diagnosis (8). Recently, CA2 was reported to facilitate transporter activity of the monocarboxylate transporter isoform 1 and 4 (MCT1/4) independent of its own catalytic activity (9,10)

Suitable for use in Western Blot. Other applications not tested.

Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recombinant protein corresponding to human CA2.

Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.

Purified by affinity chromatography.

Recognizes CA2. Species Crossreactivity: human, mouse, rat