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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP IH FC IF

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACCa is the predominant isoform in liver, adipocytes and mammary gland, while the 280kD ACCb is the major isoform in skeletal muscle and heart. Phosphorylation by AMPK at Ser79, or by PKA at Ser1200, inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.
Catalog #	A0224
Applications	Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:25
	Western Blot: 1:1000
	Immunohistochemistry (Paraffin): 1:50. Antigen retrieval with citrate buffer is recommended.
	Immunoprecipitation: 1:50
	Immunofluorescence (IF-IC): 1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity	Purified by Protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide corresponding to residues surrounding Lys557 of human acetyl CoA carboxylase a1 conjugated to KLH.
MW of Antigen	280kD.
Specificity	Recognizes endogenous levels of all isoforms of human acetyl CoA carboxylase protein at ~280kD. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.