Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibers.
Acetate Assay Kit, uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.
Key Features
Use as little as 10ul samples. Detection range: 0.20-20mM acetate for colorimetric assays and 0.13-2mM for fluorometric assays.
Applications
Direct Assays: Acetate in biological samples such as serum/plasma, in food, agriculture and environmental samples. Drug Discovery/Pharmacology: Effects of drugs on acetate metabolism.
Kit Components
A0504-85A: Assay Buffer, 1x25ml *A0504-85B: Enzyme A, 1x1vial *A0504-85C: Enzyme B, 1x1vial A0504-85D: Developer, 1x1ml A0504-85E: Dye Reagent, 1x120ul A0504-85F: ATP, 1x120ul A0504-85G: Standard, 200mM, 1x1ml
Storage and Stability
Store all components at -20°C. Kit is stable for 6 months. *A0504-85B and *A0504-85C after reconstitution stable for four weeks at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Precautions
Reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.
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