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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IP IH IF

O-GlcNAc (O-linked N-acetylglucosamine) is one of the most abundant posttranslational modifications on nuclear and cytoplasmic proteins. Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-GlcNAc residues attached to serine or threonine residues. This modification occurs via the O-GlcNAc transferase (OGT). There appears to be a competitive modification at these residues. Either the residues are glycosylated or they are phosphorylated. Because of the significance of phosphorylation in cancer research, such competition can be of major interest. O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may also obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling. Recent research has revealed that the O-GlcNAcylation of the EGF repeats of Notch are modified via a novel O-GlcNAc transferase, EOGT1.
Catalog #	A0528-19E
Applications	Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Chromatin Immunoprecipitation: 1:120-1:500
	ELISA: 1:100-1:200
	Immunofluorescence (IC): 1:100-1:200
	Immunohistochemistry (paraffin): 1:200
	Immunoprecipitation: 1:10-1:500
	Western Blot: 1:500-1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG3,k
Clone No	8J157
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid, 0.1% sodium azide.
Purity	Ascites
Immunogen	Heat killed, pepsin treated group A streptococci (Streptococcus pyrogenes)
Specificity	Recognizes beta-1,3 linked O-linked N-acetylglucosamine (O-GlcNAc) residues of streptococcal group A carbohydrate as well as O-GlcNAc glycosylated proteins
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.