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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin

Anti -Actin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IF
Catalog #A0759-06
Four muscle actins have been identified skeletal (alpha), cardiac (alpha), vascular smooth muscle (alpha) and enteric smooth muscle (gamma) actin. Also reacts with both G & F actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin.
ApplicationsSuitable for use in Indirect Immunofluorescence, Western Blot, Iodination and Solid Phase Binding. Other applications have not been tested.
Recommended DilutionIndirect immunofluorescence: 1:100; Tissue culture cells-fix with formaldehyde, treat with methanol or acetone. Glycerinated myofibrils-fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts. Cryostat sections (6um)-quick frozen in isopentane, slides treated with gelatin and formaldehyde.
Immunoblots 1:100-1:1000; On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle.
Solid Phase Binding Assay 1:800-1:1000; In an ELISA, is strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.
Optimal dilutions to be determined by the researcher.
Affinity Constant3-15 x 10e8 L/mole (gizzard > cardiac = skeletal muscle actin)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2Q1055
HostMouse
SourceChicken
ConcentrationNot determined
FormSupplied as a liquid with 0.01% sodium azide.
PurityAscites
ImmunogenPurified chicken gizzard actin
SpecificityAppears to be specific for the N-terminal two-thirds of the actin molecule (possibly near residues 50-70 in primary sequence). Reacts with every actin form tested. Species Crossreactivity: Reacts with all animal species and cell types with an actin form, including plant actin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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