| Catalog # | A0759-06 |
| Four muscle actins have been identified | skeletal (alpha), cardiac (alpha), vascular smooth muscle (alpha) and enteric smooth muscle (gamma) actin. Also reacts with both G & F actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. |
| Applications | Suitable for use in Indirect Immunofluorescence, Western Blot, Iodination and Solid Phase Binding. Other applications have not been tested. |
| Recommended Dilution | Indirect immunofluorescence: 1:100; Tissue culture cells-fix with formaldehyde, treat with methanol or acetone. Glycerinated myofibrils-fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts. Cryostat sections (6um)-quick frozen in isopentane, slides treated with gelatin and formaldehyde. |
| Immunoblots | 1:100-1:1000; On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. |
| Solid Phase Binding Assay | 1:800-1:1000; In an ELISA, is strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum. |
| Optimal dilutions to be determined by the researcher. |
| Affinity Constant | 3-15 x 10e8 L/mole (gizzard > cardiac = skeletal muscle actin) |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 2Q1055 |
| Host | Mouse |
| Source | Chicken |
| Concentration | Not determined |
| Form | Supplied as a liquid with 0.01% sodium azide. |
| Purity | Ascites |
| Immunogen | Purified chicken gizzard actin |
| Specificity | Appears to be specific for the N-terminal two-thirds of the actin molecule (possibly near residues 50-70 in primary sequence). Reacts with every actin form tested. Species Crossreactivity: Reacts with all animal species and cell types with an actin form, including plant actin. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
