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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, alpha, Smooth Muscle

Anti -Actin, alpha, Smooth Muscle

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
ASM-1 represents an excellent marker for myogenic soft
tissue tumors and smooth muscle differentiation.
Polypeptide reacting: specific for a-smooth-muscle
isoform of actin (Mr 43,000). Tumors specificallly
detected: leiomyosarcoma, leiomyoma, certain stromal
cells surrounding infiltrating ductal carcinoma of breast.
Catalog #A0760-27A
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry . Other application not tested.
Recommended DilutionImmunohistochemistry (Frozen and paraffin-embedded tissue requires protease treatment): 1:100 with PBS, pH 7.4 Incubation Time 1 hr at RT; extended with paraffin.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F160
HostMouse
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic N-terminus decapeptide of a-smooth-muscle isoform of actin.
SpecificityReccognizes myogenic soft tissue tumors and smooth muscle differentiation. Reacts on Cultured Cell lines, stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts. Species Crossreactivity: human, bovine, equine, rat, mouse and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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