Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry . Other application not tested.

Immunohistochemistry (Frozen and paraffin-embedded tissue requires protease treatment): 1:100 with PBS, pH 7.4 Incubation Time 1 hr at RT; extended with paraffin. Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic N-terminus decapeptide of a-smooth-muscle isoform of actin.

Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

Purified by Protein A affinity chromatography.

Reccognizes myogenic soft tissue tumors and smooth muscle differentiation. Reacts on Cultured Cell lines, stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts. Species Crossreactivity: human, bovine, equine, rat, mouse and chicken.