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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IF

ASM-1 represents an excellent marker for myogenic soft
tissue tumors and smooth muscle differentiation.
Polypeptide reacting: specific for a-smooth-muscle
isoform of actin (Mr 43,000). Tumors specificallly
detected: leiomyosarcoma, leiomyoma, certain stromal
cells surrounding infiltrating ductal carcinoma of breast.
Catalog #	A0760-27A
Applications	Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry . Other application not tested.
Recommended Dilution	Immunohistochemistry (Frozen and paraffin-embedded tissue requires protease treatment): 1:100 with PBS, pH 7.4 Incubation Time 1 hr at RT; extended with paraffin.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F160
Host	Mouse
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic N-terminus decapeptide of a-smooth-muscle isoform of actin.
Specificity	Reccognizes myogenic soft tissue tumors and smooth muscle differentiation. Reacts on Cultured Cell lines, stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts. Species Crossreactivity: human, bovine, equine, rat, mouse and chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.