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You are here:Home » Biologicals » Cells-Extracts, Lysates » Activating Transcription Factor 2, Control Cell Extracts (ATF2)

Activating Transcription Factor 2, Control Cell Extracts (ATF2)

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Specifications

Total cell extracts from NIH/3T3 cells prepared without treatment, suitable for use as a negative control.
Catalog #A0855-42D
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 is known to interact with a variety of viral oncoproteins and cellular tumor suppressors and has been shown to be a target of the SAPK/JNK and p38 MAP kinase signaling pathways (24). Various forms of cellular stress including genotoxic agents, inflammatory cytokines and UV irradiation stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (24). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress induced transcription by ATF-2 (24). In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
FormSupplied as a liquid in 62.5mM Tris-HCl, pH 6.8, 2% w/v SDS, 10% glycerol, 50mM DTT, 0.01% w/v bromophenol blue or phenol red.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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