ADAMTS10 belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent metalloproteases. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. Mutations in the ADAMTS10 gene may cause autosomal recessive Weill-Marchesani syndrome. The amino acid sequence of human ADAMTS10 is 100%, 96% and 94% identical to that of chimpanzee, dog and mouse.
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml of recombinant ADAMTS10 under non-reducing conditions only Immunoprecipitation: 25ug/ml from conditioned cell culture medium spiked with recombinant human ADAMTS10 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Phe26-Gly1102 from human ADAMTS10, expressed in CHO cells.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatent.
Specificity
Recognizes human ADAMTS10. In direct ELISA, does not crossreact with recombinant human ADAMTS1 3,4,5,12,13,15,16, or L2 is observed.
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