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You are here:Home » Antibodies » Abs to Receptors » Anti -Adenosine A3 Receptor (A3R)

Anti -Adenosine A3 Receptor (A3R)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified E B
Catalog #A0869-70P
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10 mg/ml for affinity pure using ECL. Based upon mRNA studies, the A3R has been found in rat testis and lung, kidney heart and brain (1). Other studies have found the mRNA to be highest in lung, spleen, and pineal glands, and to a moderate levels in brain, kidney, and testis (2). It has been found in lung, liver, kidney, and heart and very little in skeletal muscle and brain (3).
ELISA 1:10,000-50,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.1% sodium azide, 40% glycerol.
PurityPurified over the peptide-Sepharose column
ImmunogenA 15 amino acid sequence corresponding to human A3R gene (1). This region corresponds to the 3rd extracellular domain. A cysteine has been added at the NH2 terminus for coupling to KLH.
SpecificityThe human A3R peptide shows only 46% homology (7/15 AA) with rat and 20% (3/15 AA) with sheep A3R sequences. We recommend the use of A3R31 that is made to the rat A3R sequence for the detection of rat A3R. Antibody crossreactivity with various species remains to be established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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