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You are here:Home » Molecular Biology » MB-Infectious Disease » Adenovirus, Strain Adenoid 6

Adenovirus, Strain Adenoid 6

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Specifications

Adenoviridae family. A non enveloped, icosahedral virion, 75-80 nm diameter, double-stranded, linear DNA genome. Varies in clinical manifestation and severity. Symptoms include fever, rhinitis, pharyngitis, cough and conjunctivitis.
Catalog #A0876-35A
Contains high concentration of virus and viral components as well as some cellular material.
SourceAdenovirus, Strain Adenoid 6, expressed in MRC-5 cells
InactivationGamma radiation.
PurificationOptimally infected cells are disrupted by sonication and then subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated by cross flow ultrafiltration with a 30,000 molecular weight cut-off.
Applications
Suitable for use in ELISA with polystyrene and latex solid phases for detection of both IgG and IgM. Other applications not tested.
Recommended DilutionThe antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. This preparation may be further purified to meet the needs of a particular assay format.
Optimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~30kD
SourceMRC-S Cells
PurityLysate
Concentration~2.8mg/ml
FormSupplied as a liquid in MEM. No preservative added.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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