USBio Logo

A0906-92B Rabbit Anti-Adrenergic Receptor, beta 3 (B3AR, BAR3, ADRB3R, Beta-3 Adrenoreceptor) (Not for Export EU)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Serum
Applications
E
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

The beta 3-adrenergic receptor (B3AR) is a member of the super-family of G protein-coupled receptor superfamily. They are characterized by seven putative transmembrane helices connected by hydrophilic loops. The mechanism by which the activated beta ARs transmit the signals across the plasma membrane involves the stimulation of Gs. This stimulation in turn activates adenyl cyclase. Activation of adenyl cyclase yields the second messenger cAMP. B3AR is primarily present in adipocytes. The B3AR plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In humans, B3AR, a Trp to Arg replacement has recently been discovered at position 64. This change in the first coding exon has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (> 30%) and Japanese (20%). Chronic stimulation of the B3AR by highly selective B3AR agonist CL316,243, induces ectopic expression of UCP1 in adipose tissues and skeletal muscle. This expression may contribute to the potent anti-obesity effect of the beta 3-adrenergic agonist. Human B3AR is a 408aa membrane protein (rat/mouse, 400aa). The N-terminus is predicted to be extracellular; the C-terminus intracellular.

Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.
Recommended Dilution
ELISA: 1:10,000-1:100,000. Use 50-100ng of control peptide/well. See catalog #A0906-92. Western Blot (ECL): 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
A synthetic peptide corresponding to 19aa near the C-terminus of mouse Adrenergic Receptor, beta 3 (KLH). Species Sequence Homology: rat, 85%.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Serum
Specificity
Recognizes mouse Adrenergic Receptor, beta 3. Does not crossreact with human.
References
1. Barak, L.S., et al., Biochem. 34: 15,407 (1995). 2. Nahmias, C., et al., EMBO J. 10: 3721-3727 (1991). 3. Van Spronsen, A., et al., Eur. J. Biochem. 213: 1117 (1993).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved