Akt1, also known as PKBa, is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). Akt1 is frequently over-expressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).
Recombinant protein corresponding to full-length human Akt1, expressed by baculovirus in Sf 9 insect cells fused to an N-terminal GST tag. Accession Number: NM_005163.
Activity
The specific activity of Akt1 was determined to be 124 nmol/min/mg using a synthetic peptide substrate (RPRAATF).
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, Sf9 insect cells
Purity
≥70%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5ug per lane.
Form
Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 10mM glutathione, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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