Akt1, active, Recombinant, Human (Rac PKa, PKBa)
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| Akt1, also known as PKBa, is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). Akt1 is frequently over-expressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2). | | | | | | Source: | | | Recombinant full-length human Akt1 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_005163. | | | Catalog # | A1125-05N | | Activity | The specific activity of Akt1 was determined to be 124 nmol/min/mg using a synthetic peptide substrate (RPRAATF). | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 85kD | | Source | Baculovirus in Sf9 insect cells | | Purity | 90% by densitometry | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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