Akt1, PKBalpha Immunoprecipitation Kinase Assay Kit, BioAssay™
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| Used in the Immunoprecipitation and assessment of Akt 1 kinase activity from lysate. The sequence of this peptide (RPRAATF) is related to the sequence surrounding the | | | phosphorylation site of Glycogen Synthase Kinase-3 (GSK3). This peptide, which has one potential phosphorylation site, is a relatively specific substrate for Akt/PKB, because it is not phosphorylated by other protein kinases such as Rsk-2 and p70 S6 Kinase. The | | | peptide can be used to assay the activity of immunoprecipitated Akt/PKB. | | | | | | Capacity: 25 assays | | | | | | Components: | | | 5X ADBI | | | Anti-Akt/PKB PH Domain | | | Akt/SGK Speci c Peptide | | | PKA Inhibitor Peptide | | | Mg/ATP Cocktai | | | p81 Paper | | | Catalog # | A1125-15 | | Other components required but not included as part of kit are | Enzyme preparation or stimulated cell extract-containing active Akt/PKB | | Protein G Agarose | | Buffer A | 50mM Tris, pH 7.5, 1mM EDTA, 1mM EGTA, 0.5mM NaVO4, 0.1% (v/v) 2-mercaptoethanol, 1% Triton X-100, 50mM sodium fluoride, 5mM sodium pyrophosphate, 10mM sodium -glycerol phosphate, 0.1mM PMSF, 1ug/ml of aprotinin, pepstatin, leupeptin, and 1uM Microcystin | | Buffer B | 50mM Tris-HCl, pH 7.5, 0.03% (w/v) Brij-35, 0.1mM EGTA and 0.1% (v/v) 2-mercaptoethanol | | acetone vortex mixer plexiglass shielding shaking incubator timer variable volume (5-200ul) pipet + tips phosphoric acid scintillation vials scintillation fluid scintillation counter [g-32P]ATP- ~3000 Ci/mmol | | Storage and Stability | Stable for 1 year at -20°C from date of shipment. | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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