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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IP IH

Aminopeptidase O (AP-O; also C9orf3) is a 93kD (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N-terminal Arg and Asn residues. Human AP-O is 819 amino acids in length. It contains one catalytic domain (aa 167-589) with a Zn-binding site (His479-His483) and a C-terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455-553; a second has an 11 aa substitution for the C-terminal 265 amino acids; and a third shows both a deletion of aa 455-553 and an 11 aa substitution for the C-terminal 46 amino acids.
Catalog #	A1379-47
Applications	Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Direct ELISA: 0.5-1.0ug/ml. Detection limit for rhAminopeptidase O is ~0.3ng/well.
	Western Blot: 0.1-0.2ug/ml. Detection limit for rhAminopeptidase O is ~5ng/lane under non-reducing and reducing conditions.
	Immunoprecipitation: Used to precipitate rhAminopeptidase O from Sf21 lysates.
	Immunohistochemistry: 3-10ug/ml. Antigen retrieval is recommended.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Purified Sf 21-derived rhAminopeptidase O isoform 1 (aa 1-819).
Specificity	Recognizes human Aminopeptidase O. Species sequence homology: mouse, 76%.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.