Aminopeptidase O (AP-O; also C9orf3) is a 93kD (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N-terminal Arg and Asn residues. Human AP-O is 819 amino acids in length. It contains one catalytic domain (aa 167-589) with a Zn-binding site (His479-His483) and a C-terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455-553; a second has an 11 aa substitution for the C-terminal 265 amino acids; and a third shows both a deletion of aa 455-553 and an 11 aa substitution for the C-terminal 46 amino acids.
Applications
Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1ug/ml using recombinant Human Aminopeptidase O/ONPEP Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with Recombinant Human Aminopeptidase O/ONPEP, Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human placenta. Antigen retrieval is recommended. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corrresponding to Met1-Phe819 from Aminopeptidase O isoform 1, expressed in Sf 21 cells. Uniprot/Swiss Accession: Q8N6M6. Species sequence homology: mouse, 76%.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Aminopeptidase O.
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