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A2275-50 β-Amylase, Suspension (Sweet Potato) CAS: 9000-91-3

Specifications
References
CAS Number
9000-91-3
Grade
Molecular Biology Grade
MDL Number
MFCD00081391
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
EC=3.2.1.2; 1,4-a-D-Glucan maltohydrolase; EC 3.2.1.2

Exoenzyme that releases successive maltose units from the nonreducing end of a polysaccharide chain by hydrolysis of a 1,4-glucan linkages. The shortest normal saccharide attacked is maltotetraose. Since it is unable to bypass branch linkages in branched polysaccharides such as glycogen or amylopectin, the hydrolysis is incomplete and a macromolecular limit dextrin remains.

Found primarily in the seeds of higher plants and sweet potatoes. It yields a single product: maltose. The enzyme is useful in structural studies of starch and glycogen.
Source
Sweet potato
CAS No
9000-91-3
Form
Supplied as an ammonium sulfate suspension
Protein by UV Absorbance
As Reported
Units/mg protein
≥750 units/mg protein (E1%/280)
Unit Definition
1 unit releases 1mg of beta-maltose from starch in 3 minutes at 20ºC, pH 4.8.
Storage and Stability
May be stored at 4°C. Do not freeze. Stable for 12 months after receipt.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Abdullah, M., Lee, E., and Whelan, W.: An Enzymic Method for the Microdetermination of the Average Unit-Chain Lengths of Glycogen and Amylopectin, Biochem. J., 97, 10 (1965). 2. Balls, A., Walden, M., and Thompson, R.: A Crystalline [[beta]]-Amylase from Sweet Potatoes, J. Biol. Chem., 173, 9 (1948). 3. Banks, W., and Greenwood, C.: Comments on "Enzymic Methods for the Microdetermination of Glycogen and Amylopectin, and Their Unit Chain-Lengths", Arch. Biochem. Biophys., 136, 320 (1970). 4. Bernfeld, P.: Amylases, [[alpha]] and [[beta]], in Methods in Enzymology, I, (Colowick, S., and Kaplan, N., eds.), Academic Press, NY, 149 (1955). 5. Carter, J., and Lee, E.: An Enzymic Method for Determination of Average Chain Lengths of Glycogens and Amylopectins, Anal. Biochem., 39, 373 (1971). 6. Ceska, M.: A New Type of Reagent for the Detection of Molecular Varieties of Some Hydrolytic Enzymes: Detection of [[beta]]-Amylase Isoenzymes, Biochem. J., 121, 575 (1971). 7. Chapman, G., Pallas, J., and Mendicino, J.: The Hydrolysis of Maltodextrins by a [[beta]]-Amylase Isolated from Leaves of Vicia faba, Biochim. Biophys. Acta, 276, 491 (1972). 8. Colman, P., and Matthews, B.: Symmetry, Molecular Weight and Crystallographic Data for Sweet Potato [[beta]]-Amylase, J. Mol. Biol., 60, 163 (1971). 9. Englard, S., and Singer, T.: Physicochemical Studies on [[beta]]-Amylase, J. Biol. Chem., 187, 213 (1950). 10. Francofraguas, L., and Batistaviera, F.: Thiophilic Interaction Chromatography of Sweet Potato [[beta]]-Amylase, J. Chromatography, 604, 103 (1992). 11. French, D.: [[beta]]-Amylases in The Enzymes, IV, 2nd ed., (Boyer, P., Lardy, H., and Myrback, K., eds.), Academic Press, NY, 345 (1960). 12. Hehre, E, Brewer, C., and Genghof, D.: Scope and Mechanism of Carbohydrase Action. Hydrolytic and Nonhydrolytic Action of [[beta]]-Amylase on [[alpha]] and [[beta]] Maltosyl Fluoride, J. Biol. Chem., 254, 5942 (1979). 13. Henis, Y., Yaron, T., Lamed, R., Rishpon, J., Sahar, E., and Katchalski-Katzir, E.: Mobility of Enzymes on Insoluble Substrates: The [[beta]]-Amylase-Starch Gel System, Biopolymers, 27, 123 (1988). 14. Kainuma, K., and French, D.: Action of Pancreatic Alpha-Amylase and Sweet Potato Beta-Amylase on 6-2 and 6-3-[[alpha]]-Glucosylmatol-Oliogsaccharides, FEBS Lett., 6, 182 (1970). 15. Kato, M., Hiromi, K., and Morita, Y.: Purification and Kinetic Studies of Wheat Bran [[beta]]-Amylase. Evaluation of Subsite Affinities, J. Biochem. Japan, 75, 563 (1974). 16. Kohno, A., Nanmori, T., and Shinke, R.: Purification of [[beta]]-Amylase from Alfalfa (Medicago sativa L.) Seeds, J. Biochem. Japan, 105, 231 (1989). 17. Lee, E.: The Action of Sweet Potato [[beta]]-Amylase on Glycogen and Amylopectin: Formation of a Novel Limit Dextrin, Arch. Biochem. Biophys., 146, 488 (1971). 18. Lee, E., and Whelan, W.: An Enzymic Impurity in Crystalline Sweet-Potato [[beta]]-Amylase, Biochem. J., 95, 27 (1965). 19. Marshall, J., and Whelan, W.: Removal of [[beta]]-Glucosidase Impurity from Crystalline Sweet-Potato [[beta]] Amylase, Anal. Biochem., 52, 642 (1973). 20. Mikami, B., Morita, Y., and Fukazawa, C.: Primary Structure and Function of [[beta]]-amylase, Seikagaku, 60, 211 (1988). 21. Misra, U., and French, D.: Inhibition and Action Pattern of [[beta]]-Amylase in Presence of Maltose, Biochem. J., 77, 1P (1960). 22. Morita, Y., Aibara, S., Yamashita, H., Yagi, F., Suganuma, T., and Hiromi, K.: Crystallization and Preliminary X-Ray Investigation of Soybean [[beta]]-Amylase, J. Biochem. Japan, 77, 343 (1975). 23. Myrback, K., and Neumuller, G.: Amylases and the Hydrolysis of Starch and Glycogen, in The Enzymes, I, Part 1, (Sumner, J., and Myrback, K., eds.), Academic Press, NY, 653 (1950). 24. Nakayama, S., and Amagase, S.: An Improved Method for the Purification of Sweet Potato [[beta]]-Amylase, J. Biochem. Japan, 54, 375 (1963). 25. Nipkow, A., Shen, G., and Zeikus, J.: Continuous Production of Thermostable [[beta]]-Amylase with Clostridium thermosulfurogenes: Effect of Culture Conditions and Metabolite Levels on Enzyme Synthesis and Activity, Appl. Env. Micro., 555, 689 (1989). 26. Nummi, M., Niku-Paavola, M., and Enzri, T.: Isoelectric Properties of Barley [[beta]]-Amylase, Acta Chem. Scand., 26, 1711 (1972). 27. Rowe, A., and Weill, C.: The Inhibition of [[beta]]-Amylase by Ascorbic Acid, J. Am. Chem. Soc., 81, 921 (1959). 28. Rowe, A., and Weill, C.: The Inhibition of [[beta]]-Amylase by Ascorbic Acid, Biochim. Biophys. Acta, 65, 245 (1962). 29. Saha, B., Lecureux, L., and Zeikus, J.: Raw Starch Adsorption-Desorption Purification of a Thermostable [[beta]]-Amylase from Clostridium thermosulfurogenes, Anal. Biochem., 175, 569 (1988). 30. Shinke, R., Kunimi, Y., and Nishira, H.: Isolation and Characterization of [[beta]]-Amylase Producing Microorganisms, J. Ferm. Techn., 53, 687 (1975). 31. Shinke, R., Kunimi, Y., and Nishira, H.: Production and Some Properties of [[beta]]-Amylase of Bacillus sp. BQ10, J. Ferm. Tech., 53, 693 (1975). 32. Shinke, R., Kunimi, Y., and Nishira, H.: Production and Some Enzymatic Properties of [[beta]]-Amylase of Pseudomonas sp. BQ6, J. Ferm. Tech., 53, 698 (1975). 33. Spradlin, J., and Thoma, J.: [[beta]]-Amylase Thiol Groups. Possible Regulator Sites, J. Biol. Chem., 245, 117 (1970). 34. Spradlin, J., Thoma, J., and Filmer, D.: Beta Amylase Thiol Groups. Possible Regulator Sites?, Arch. Biochem. Biophys., 134, 262 (1969). 35. Subbaramaiah, K., and Sharma, R.: Characterization of [[beta]]-Amylase from Sinapsis alba Cotyledons, Phytochem., 29, 1417 (1990). 36. Takeda, Y., and Hizukuri, S.: Improved Method for Crystallization of Sweet Potato [[beta]]-Amylase, Biochim. Biophys. Acta, 185, 469 (1969). 37. Takeda, Y., and Hizukuri, S.: Effect of Triton X-100 on Sweet Potato [[beta]]-Amylase, Biochim. Biophys. Acta, 268, 175 (1972). 38. Thoma, J.: Interaction of [[beta]]-Amylase with Substrates and Inhibitors with Comments on Koshland's Induced-Fit Theory. A Reply, Eur. J. Biochem., 44, 139 (1974). 39. Thoma, J., and Koshland, D.: Stereochemistry of Enzyme, Substrate, and Products During [[beta]]-Amylase Action, J. Biol. Chem., 235, 2511 (1960). 40. Thoma, J., Brothers, C., and Spradlin, J.: Subsite Mapping of Enzymes. Studies on Bacillus subtilis Amylase, Biochem., 9, 1768 (1970). 41. Thoma, J., Koshland, D., Ruscica, J., and Baldwin, R.: The Number of Binding Sites of Sweet Potato Beta Amylase, Biochem. Biophys. Res. Comm., 12, 184 (1963). 42. Thoma, J., Koshland, D. Shinke, R., and Ruscica, J.: Influence of Sulfhydryl Groups on the Activity of Sweet Potato Beta-Amylase, Biochem., 4, 714 (1965). 43. Thoma, J., Spradlin, J., and Dygert, S.: Plant and Animal Amylases, in The Enzymes, V, 3rd ed., (Boyer, P., ed.), Academic Press, NY, 115 (1971). 44. Uehara, K., Mizoguchi, T., Kishida, K., and Mannen, S.: Studies on Sweet Potato [[beta]]-Amylase. II. Susceptibility of Tryptophan to the Riboflavin-Sensitized Photooxidation in the Presence of Adenine, J. Biochem. Japan, 69, 27 (1971). 45. Visuri, K., and Nummi, M.: Purification and Characterization of Crystalline [[beta]]-Amylase from Barley, Eur. J. Biochem., 28, 555 (1972). 46. Vretblad, P.: Biospecific Affinity Chromatography of Sweet-Potato Beta-Amylase, Biochem. Soc. Trans., 2, 1327 (1974).
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