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You are here:Home » Antibodies » Antibodies-Receptors » Anti -Androgen Receptor (AR)

Anti -Androgen Receptor (AR)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP IH IC IF
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
Catalog #A2281-10
Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Gel Super Shift, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunofluorescence/Immunocytochemistry: 5ug/ml
Immunohistochemistry (frozen and paraffin): 4ug/ml
Western Blot: 1ug/ml. Detects an ~110kD protein representing androgen receptor in Human tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with this antibody results in intense nuclear staining.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4i297
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.05% sodium azide. Reconstitute with 50ul sterile PBS.
PurityPurified by Protein A affinity chromatography.
ImmunogenA 241 amino acid fusion protein derived from human androgen receptor. Cellular location: nuclear.
SpecificityRecognizes human Androgen receptor. Does not crossreact with estrogen, progesterone or glucocorticoid receptors. Species Crossreactivity: rat, mouse and primate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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