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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified B IP IH IC IF

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Androgen receptors (AR) have been found in a variety of tissues, including reproductive organs, central nervous system and skeletal muscle.
Catalog #	A2281-10
Applications
	Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Gel Super Shift, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Immunofluorescence/Immunocytochemistry: 5ug/ml
	Immunohistochemistry (frozen and paraffin): 4ug/ml
	Western Blot: 1ug/ml. Detects an ~110kD protein representing androgen receptor in Human tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with this antibody results in intense nuclear staining.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG
Clone No	4i297
Host	Rat
Source	Human
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.05% sodium azide. Reconstitute with 50ul sterile PBS.
Purity	Purified by Protein A affinity chromatography.
Immunogen	A 241 amino acid fusion protein derived from human androgen receptor. Cellular location: nuclear.
Specificity	Recognizes human Androgen receptor. Does not crossreact with estrogen, progesterone or glucocorticoid receptors. Species Crossreactivity: rat, mouse and primate.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.