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You are here:Home » Antibodies » Abs to Enzymes, Angiotensin Converting (ACE) » Anti -Angiotensin Converting Enzyme 2 (ACE-2, ACEH)

Anti -Angiotensin Converting Enzyme 2 (ACE-2, ACEH)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Renin-Angiotensin System (RAS) is a critical regulator of blood pressure homeostasis. The protease renin cleaves angiotensinogen into inactive decameric peptide angiotensin-I (Ang-I). Angiotensin-converting enzyme (ACE) then cleaves a C-terminal dipeptide from Ang-I to form an active octamer angiotensin-II (Ang-II), which can contribute to hypertension by promoting vascular smooth muscle vasoconstriction and renal tubule sodium reabsorption. ACE mutant mice display spontaneous hypotension, partial male infertility and kidney malformations. ACE is found in somatic (s-ACE) and testicular/germinal (t-ACE) isoforms.
The ACE-2 gene has been mapped at human chromosome Xp22. ACE-2 enzymes from human (805aa) and mouse (798aa) are single chain proteins with 40% seq homology to N- and C-terminal domains of ACE. However, in contrast to s-ACE, which consists of two catalytic sites, ACE-2 contains only one active site. Unlike s-ACE and t-ACE, which are dipeptidyl-carboxypeptidases, ACE-2 acts as a carboxypeptidase, cleaving a single residue from Ang-I, generating Ang1-9 and a single residue from Ang-II to generate Ang1-7. ACE-2 can cleave angiotensin-I but not bradykinin and the enzyme activity is not inhibited by the ACE inhibitors. This enzyme is expressed highly in heart, kidney and testis, and moderately in colon, small intestine and ovary. ACE-2 is an essential regulator of heart function because targeted disruption of this enzyme in mice results in severe cardiac contractility defect, increased angiotensin-II levels and upregulation of hypoxia-induced genes in the heart.
Catalog #A2295-01W9
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionELISA: 1:10K-1:30K
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by researcher.
Positive ControlA2295-01J1, Angiotensin Converting Enzyme 2, Recombinant, Human
HybridomaPurified NSO-derived recombinant human angiotensin I converting enzyme-2 (rhACE-2) ectodomain (aa18-740).
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7C9
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified NSO-derived recombinant human angiotensin I converting enzyme-2 (rhACE-2) ectodomain (aa18-740).
SpecificityRecognizes human ACE-2. Does not crossreact with ACE-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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