Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF-kB, and p53.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Pro2-Leu318 from human APE, expressed in E. coli.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human APE. Species Crossreactivity: Mouse and rat.