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You are here:Home » Antibodies » Abs to Enzymes, Endonuclease » Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)

Anti -APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF-kB, and p53.
Catalog #A2298-74A1
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS or sterile 40-50% glycerol.
PurityPurified by human Wnt-6 affinity chromatography.
ImmunogenE. coli-derived recombinant human APE (aa2-318) GenBankAccession #NM_001641
SpecificityRecognizes human APE. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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