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A2299-76D Mouse Anti-Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P02649
Isotype
IgG1,k
Clone Number
2B16 (E6D7)
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Gene ID
348
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

Applications
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of
Western Blot: 1:1000-1:10,000 Immunohistochemistry (Frozen, FFPE): 1:1000-1:10,000 1. Formic acid (70%) for 10-30 minutes at RT. 2. HIER (Heat Induced Epitope Retrieval) with buffers such as citrate. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Apo E. Recognizes E2, E3 and E4 isoforms of apolipoprotein E. Does not react with mouse and rabbit.
References
1. Norman, R.R., et al., “Apolipoprotein E genotyping in Alzheimer’s Disease”, Ann. N.Y. Acad. Science, 801 (Reviews). 2. Munoz, D.G., et al., (2000), CMAJ, 162(1): 65–72. 3. Tomiyama, T., et al., (1999),Cell Mol. Life Sci., 6(3-4): 268–279. 4. Hagberg, J.M., et al., (2000), Physiol. Genomics, 4(2): 101-–108. 5. Saunders, A.M., (2000), J. Neuropathol. Exp. Neurol., 59(9): 751–758. 6. Golabek, A.A., et al., (2000), Biophys. J. 79(2): 1008–1015. 7. Golabek, A.A., et al., (1996), J. Biol. Chem., 271(18): 10602–10606. 8. Zunarelli, E., et al., (2000), Breast Cancer Res. Treat, 63(3): 193–198. 9. Zunarelli, E., et al., (2000), Clin Neuropathol., 19(1): 1–6.
USBio References
No references available
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