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Anti -Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4)
Anti -Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH |
|
| Catalog # | A2299-76D | | Applications | Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Western Blot: 1:1000-1:10,000 | | Immunohistochemistry (Frozen and formalin-fixed paraffin-embedded tissues): 1:1000-1:10,000 | | The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of: | | 1. Formic acid (70%) for 10-30 minutes at RT. | | 2. HIER (Heat Induced Epitope Retrieval) with buffers. | | Optimal dilutions to be determined by the researcher. | | Source | Tissue culture supernatant | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 2B16 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. No preservative added. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide spanning the polymorphic aa position 158 of Apo E. | | Specificity | Recognizes human Apo E. Recognizes E2, E3 and E4 isoforms of apolipoprotein E. Does not react with mouse and rabbit. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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