Apolipoprotein E,E2,E3,E4

Clone Type

Monoclonal

Host

Mouse

Source

Human

Swiss Prot

P02649

Isotype

IgG1,k

Clone Number

2B16 (E6D7)

Grade

Affinity Purified

Applications

Crossreactivity

Gene ID

348

Shipping Temp

Blue Ice

Storage Temp

-20°C

Notes

Preservative Free

Applications

Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of

Western Blot: 1:1000-1:10,000 Immunohistochemistry (Frozen, FFPE): 1:1000-1:10,000 1. Formic acid (70%) for 10-30 minutes at RT. 2. HIER (Heat Induced Epitope Retrieval) with buffers such as citrate. Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes human Apo E. Recognizes E2, E3 and E4 isoforms of apolipoprotein E. Does not react with mouse and rabbit.

References

1. Norman, R.R., et al., “Apolipoprotein E genotyping in Alzheimer’s Disease”, Ann. N.Y. Acad. Science, 801 (Reviews). 2. Munoz, D.G., et al., (2000), CMAJ, 162(1): 65–72. 3. Tomiyama, T., et al., (1999),Cell Mol. Life Sci., 6(3-4): 268–279. 4. Hagberg, J.M., et al., (2000), Physiol. Genomics, 4(2): 101-–108. 5. Saunders, A.M., (2000), J. Neuropathol. Exp. Neurol., 59(9): 751–758. 6. Golabek, A.A., et al., (2000), Biophys. J. 79(2): 1008–1015. 7. Golabek, A.A., et al., (1996), J. Biol. Chem., 271(18): 10602–10606. 8. Zunarelli, E., et al., (2000), Breast Cancer Res. Treat, 63(3): 193–198. 9. Zunarelli, E., et al., (2000), Clin Neuropathol., 19(1): 1–6.

USBio References

No references available

Product	Size	List	Your Price	Qty	Ext Price
Subtotal:	Subtotal:
Subtotal:	Subtotal:
Total Coupon Savings:	Total Coupon Savings:	()
Your cart is currently empty.
- Coupon Code

Product

Size

List

Your Price

Qty

Ext Price

Subtotal:

Total Coupon Savings:

()

Your cart is currently empty.

- Coupon Code

A2299-76D Mouse Anti-Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4)

Clone Type

Host

Source

Swiss Prot

Isotype

Clone Number

Grade

Applications

Crossreactivity

Gene ID

Shipping Temp

Storage Temp

Notes

References

USBio References

Conjugates

Pricing

Order

Cart Summary

Recently Viewed

Special Offers

Contact Us

Site Map

Technical Resources

Distributors

Payment Methods