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You are here:Home » Antibodies » Abs to Lipoproteins » Anti -Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4)

Anti -Apolipoprotein E,E2,E3,E4 (Apo E,E2,E3,E4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
Catalog #A2299-76D
ApplicationsSuitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:1000-1:10,000
Immunohistochemistry (Frozen and formalin-fixed paraffin-embedded tissues): 1:1000-1:10,000
The staining intensity of formalin-fixed paraffin embedded tissues may be significantly improved by the pretreatment of:
1. Formic acid (70%) for 10-30 minutes at RT.
2. HIER (Heat Induced Epitope Retrieval) with buffers.
Optimal dilutions to be determined by the researcher.
SourceTissue culture supernatant
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG1
Clone No2B16
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide spanning the polymorphic aa position 158 of Apo E.
SpecificityRecognizes human Apo E. Recognizes E2, E3 and E4 isoforms of apolipoprotein E. Does not react with mouse and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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