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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IF

The INK4a-ARF locus encodes two unrelated proteins both of which function in tumor suppression. p14ARF arrests the cell cycle in a p53-dependent manner. p14ARF binds to mdm2 and promotes the rapid degradation of mdm2 protein. This interaction is mediated by the exon 1b-encoded N-terminal domain of p14ARF and a C-terminal region of mdm2. ARF-mediated mdm2 degradation is associated with mdm2 modification and concurrent p53 stabilization and accumulation. Deletion of the ARF-INK4a locus simultaneously impairs both the INK4a-cyclin D/cdk4-RB and the ARF-mdm2-p53 pathways.
Catalog #	A3330-02
Positive Control	HeLa cells.
Applications and Suggested Dilutions	Immunofluorescence
	Immunoprecipitation (Use Protein G)
	(Ab 2mg/mg protein lysate)
	Western Blotting (Ab 2-4mg/ml for 2hrs at RT)
Cellular Localization	Nuclear
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.479
Host	Mouse
Source	Human
Concentration	0.2mg/ml
Form	10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant p14ARF protein.
Specificity	Recognizes human p14ARF. Does not react with mouse and rat.
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.