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Anti -ARG1 (Arginase 1, AI type I arginase, arginase 1 liver) (Carboxyfluorescein)
Anti -ARG1 (Arginase 1, AI type I arginase, arginase 1 liver) (Carboxyfluorescein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
FC |
|
| Arginase 1 (ARG1) is a 35-40kD member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly down-regulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human ARG1 is 322aa in length. Its enzyme region comprises aa9-309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105kD homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa204-289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1. | | | Catalog # | A3340-14R | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Absorption | 488nm | | Emission | 515-545nm | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. CFS conjugates are sensitive to light. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with Carboxyfluorescein (CFS). | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa1-322 from human ARG1 expressed in E. coli (P05089). | | Specificity | Recognizes human ARG1. Species sequence homology: mouse and rat (87%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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