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A3340-14R Sheep Anti-ARG1 (Arginase 1, AI type I arginase, arginase 1 liver) (Carboxyfluorescein)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
P05089
Conjugate
Carboxyfluorescein
Isotype
IgG
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Arginase 1 (ARG1) is a 35-40kD member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly down-regulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human ARG1 is 322aa in length. Its enzyme region comprises aa9-309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105kD homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa204-289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry (intracellular staining); Neat. Use 10ul per 10^6 cells. Optimal dilutions to be determined by the researcher.
Absorption: 488nm
Emission: 515-545nm
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. CFS conjugates are sensitive to light.
Immunogen
Recombinant corresponding to aa1-322 from human ARG1 expressed in E. coli (P05089).
Form
Supplied as a liquid in 1ml saline, 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein (CFS).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ARG1. Species Cross-reactivity: mouse and rat.
Conjugates
Pricing
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