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You are here:Home » Antibodies » Abs to Enzymes » Anti -ARG1 (Arginase 1, AI type I arginase, arginase 1 liver) (Carboxyfluorescein)

Anti -ARG1 (Arginase 1, AI type I arginase, arginase 1 liver) (Carboxyfluorescein)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified FC
Arginase 1 (ARG1) is a 35-40kD member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly down-regulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human ARG1 is 322aa in length. Its enzyme region comprises aa9-309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105kD homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa204-289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1.
Catalog #A3340-14R
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Absorption 488nm
Emission 515-545nm
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. CFS conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with Carboxyfluorescein (CFS).
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa1-322 from human ARG1 expressed in E. coli (P05089).
SpecificityRecognizes human ARG1. Species sequence homology: mouse and rat (87%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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