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Anti -Arg-X-Tyr, Phe-X Motif, (Ser) phosphorylated
Anti -Arg-X-Tyr, Phe-X Motif, (Ser) phosphorylated
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Binding of 14-3-3 is mediated through phospho-serine-containing protein. Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP. Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR. Even though Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. | | | Catalog # | A3551 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | ELISA: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. | | Purity | Purified by Protein A and immunoaffinity chromatography. | | Immunogen | Synthetic phospho- (Ser) Arg-X-Tyr/Phe-X-pSer–containing peptides. | | Specificity | Detects endogenous levels of proteins containing the phospho-(Ser) Arg-XTyr/ Phe-X-pSer motif. This antibody is highly specific to phosphorylated Arg-X-Tyr/Phe-X-pSer–containing peptides and proteins and does not recognize non-phosphorylated serine or phospho-threonine proteins containing the same motif or other phospho-serine/threonine-containing proteins and peptides without this motif. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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