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You are here:Home » Antibodies » Abs to Proteins » Anti -Arg-X-Tyr, Phe-X Motif, (Ser) phosphorylated

Anti -Arg-X-Tyr, Phe-X Motif, (Ser) phosphorylated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Binding of 14-3-3 is mediated through phospho-serine-containing protein. Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP. Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR. Even though Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif.
Catalog #A3551
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
PurityPurified by Protein A and immunoaffinity chromatography.
ImmunogenSynthetic phospho- (Ser) Arg-X-Tyr/Phe-X-pSer–containing peptides.
SpecificityDetects endogenous levels of proteins containing the phospho-(Ser) Arg-XTyr/ Phe-X-pSer motif. This antibody is highly specific to phosphorylated Arg-X-Tyr/Phe-X-pSer–containing peptides and proteins and does not recognize non-phosphorylated serine or phospho-threonine proteins containing the same motif or other phospho-serine/threonine-containing proteins and peptides without this motif.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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