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A3885-10 Mouse Anti-Astrocytes

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
J1-31
Grade
Ascites
Applications
IC IHC WB
Crossreactivity
Gp Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

By immunofluorescence microscopy, A3885-10 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, A3885-10 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}.

Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human cerebral white matter from a multiple sclerosis patient.
Form
Supplied as a liquid.
Purity
Ascites
Specificity
Recognizes an intracellular protein antigen (MW 30kD) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS). The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue and is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed). Species Crossreactivity: guinea pig, human, mouse and rat
References
1) Singh, R., Singh, B., & Malhotra, S.K. 1985. A new marker protein for astrocytes. Bioscience Reports, Vol 6, pp 73-80, 1985. 2) Predy, R., Malhotra, S.K..Das, G.D. Enhanced expression of a protein antigen (JI-31 antigen, 30 Kilodaltons) by reactive astrocytes in lacerated spinal cord. J. Neuroscience Research, Vol. 19, 397-404, 1988. 3) Malhotra, S.K., Predy, R., Johnson, E.S., Singh, R., & Leeuw, K. Novel astrocytic protein in multiple sclerosis plaques. J. Neuroscience Research, Vol 21, 36-49, 1989. 4) Malhotra, S.K., Svensson, M., Aldskogius, H., Bhatnagar, R., Das, G.D., & Shnitka, T.K. Diversity amongst reactive astrocytes: proximal reactive astrocytes in lacerated spinal cord preferentially react with monoclonal antibody JI-31. Brain Research Bulletin, 10, 3395-404, 1993. 5) Malhotra, S.K., Luong, L.T., Bhatnagar, R., & Shnitka, T.K.,Upregulation of reactive astrogliosis in the rat glioma cell line by combined mechanical and chemical injuries. Cytobios, 88, 115-134, 1997.
USBio References
No references available
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