Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B

p97 ATPase is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p.
Catalog #	A4001-10
Applications	Suitable for use in Immunoblot. Not suitable for use in Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Immunoblot: 1:1000-1:2000. Dilution Buffer: 0.15M PBS, 0.1% BSA, 0.1% sodium azide (ECL: 5% milk powder in TBST)
	Optimal dilutions to be determined by the researcher.
Positive Control	Protein extracts from all vertebrate tissues and cells.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F125
Host	Mouse
Source	Xenopus
Concentration	Not Determined
Form	Supplied as a liquid.
Purity	Supernatant
Immunogen	15S Mg2+ ATPase complex purified from Xenopus laevis ovary.
Specificity	Recognizes Xenopus ATPase, p97. Reacts with p97 subunit of 15S Mg2+-ATPase. Species Crossreactivity: rabbit, rat, human, vertebrate cells and tissues. Does not crossreact with the corresponding protein in budding yeast (Cdc48p).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.