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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Axin1 (AXIN1, AXIN, Axis Inhibition Protein 1, MGC5231)

Anti -Axin1 (AXIN1, AXIN, Axis Inhibition Protein 1, MGC5231)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.
Catalog #A4747-01
ApplicationsSuitable for use in Immunoprecipitation/Western Blot. Other applications not tested.
Recommended DilutionsImmunoprecipitation (WB): 1ug/ml detected Axin-1 immunoprecipitated from 10ug of SW-480 cell lystate.
Optimal dilutions to be determined by the researcher.
Positive ControlSW-480 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No10F631
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1. Species Sequence Homology: mouse
SpecificityRecognizes human Axin-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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