Suitable for use in ELISA, Immunoprecipitation and Neutralization. Other applications not tested.

ELISA: Use in a Direct ELISA. Immunoprecipitation: 25ug/ml to immunoprecipitate BACE-1 from conditioned media of cell culture spike with recombinant human BACE1 or recombinant mouse BACE1. Western Blot after Immunoprecipitation. Neutralization: The Neutralization Dose (ND50) is typically 10ug/ml. It is measured by the ability of the antibody to neutralize recombinant human BACE1 or recombinant mouse BACE1 cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10uM). Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Thr22-Tyr460 of human BACE1 expressed in NS0 cells.

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)

Recognizes human BACE1 ectodomain. |Species Crossreactvity: mouse. Does not react with recombinant human or mouse BACE2, recombinant human ADAM8, ADAM9, ADAM10, ADAM15 or TACE.