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You are here:Home » Antibodies » Abs to Apoptosis » Anti -BACE1, Ectodomain (Beta-site APP Cleaving Enzyme)

Anti -BACE1, Ectodomain (Beta-site APP Cleaving Enzyme)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP N
Cleavage of amyloid precursor protein (APP) into 40 or 42 amino acid amyloid beta (ab) peptides is a result of the action of two proteases beta- and gamma-secretase. The beta-site APP cleaving enzyme, or BACE1, is a transmembrane aspartic protease that is localized in the golgi and endosomes. Low levels of BACE1 mRNA are found in most adult tissues with a higher level of expression in pancreas.
Catalog #B0002-91L
ApplicationsSuitable for use in ELISA, Immunoprecipitation and Neutralization. Other applications not tested.
Recommended DilutionsELISA: Use in a Direct ELISA.
Immunoprecipitation: 25ug/ml to immunoprecipitate BACE-1 from conditioned media of cell culture spike with recombinant human BACE1 or recombinant mouse BACE1. Western Blot after Immunoprecipitation.
Neutralization: The Neutralization Dose (ND50) is typically 10ug/ml. It is measured by the ability of the antibody to neutralize recombinant human BACE1 or recombinant mouse BACE1 cleavage of the fluorogenic peptide substrate Mca-SEVNLDAEFRK(Dnp)RR-NH2 (10uM).
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8H106
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
ImmunogenRecombinant protein corresponding to aa22-460 of human BACE1 expressed in NOS cells (P56817).
SpecificityRecognizes human BACE1 ectodomain. Species Crossreactvity: mouse. Does not react with recombinant human or mouse BACE2, recombinant human ADAM8, ADAM9, ADAM10, ADAM15 or TACE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesASP2, BACE, EC=3.4.23.46, FLJ90568, HSPC104, KIAA1149, Memapsin-2


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