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B0003 Rabbit Anti-BACE2, Asp1 (NT) (Beta-site APP Cleaving Enzyme, Aspartyl Protease 2, DRAP, Down Region Aspartic Protease, Memapsin)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor (APP) at b-and g-sites by proteases. The long-sought b-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1–4). A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1 (4–9). BACE2 also cleaves APP at b-site and at a different site within Ab (8). BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and Ab may also contribute to the pathogenesis of Down syndrome (6,7)

Applications
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution
Western Blotting: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control: Human heart tissue lysate can be used as positive control.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Raised against a synthetic peptide (APTPGPGTPAERHADG) corresponding to amino acids 44 to 59 of human BACE2 (4).
Form
Supplied as a liquid, 100ug in 200ul of PBS, 0.02% sodium azide.
Purity
Immunoaffinity chromatography purified IgG
Specificity
Recognizes human BACE2. Species crossreactivity: Mouse and rat.
References
1. Vassar R, et al. Beta-secretase cleavage of Alzheimer’s amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735–41 2. Hussain I, et al. Identification of a novel aspartic protease (Asp 2) as beta-secretase. Mol Cell Neurosci 1999;14:419–27 3. Sinha S, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999;402:537–40 4. Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer’s disease beta-secretase activity. Nature 1999;402:533–7 5. Lin X, et al. Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein. Proc Natl Acad Sci USA 2000 15;97:1456–60 6. Acquati F, et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett 2000;468:59–64 7. Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer’s amyloid precursor protein beta-secretase. Cytogenet Cell Genet 2000;89:177–184 8. Farzan M, et al. BACE2, a beta-secretase homolog, cleaves at the beta site and within the amyloid-beta region of the amyloid-beta precursor protein. Proc Natl Acad Sci USA 2000;97:9712–7 9. Bennett BD, et al. Expression analysis of BACE2 in brain and peripheral tissues. J Biol Chem 2000;275:20647–51
USBio References
No references available
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