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You are here:Home » Molecular Biology » Growth Factors-BAFF » BAFF CD8a, Fusion Protein (Preservative Free)

BAFF CD8a, Fusion Protein (Preservative Free)

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Specifications

The human B cell activating factor (BAFF) and APRIL(a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: TACI (transmembrane activator and CAML-interactor) and BCMA (B cell maturation antigen), both of which are restricted to B cells(3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). Recently a third receptor for BAFF (BAFF-R) was described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFF-R may the primary receptor for BAFF in mice(8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren's Syndrome(11). Recombinant BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR.
Catalog #B0003-67EX
Functional Application Human BAFF(trn)-muCD8 binds to human cell surface TACI/BCMA/BAFFR .
Human BAFFtrn(TALL-1, Blys, THANK)-muCD8 Fusion Protein
Molecular Structure A soluble molecule consisting of 151 (VQGPEE...c-term) aminoacids of the extracellular domain of human BAFF fused to the extracellular domain (167aa) of murine CD8 alpha, with a predicted monomeric weight of 38.5 kd. A portion of the extracellular region of BAFF was truncated to eliminate a potential protease cleavage site.
Transfectant Cell Line CHO
ApplicationsSuitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight38.5kD
SourceCHO cells
PurityPurified by affinity chromatography.
Concentration~0.5mg/ml
FormSupplied as a liquid in 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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