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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Barmotin (Tight Juncion-Associated Protein)

Anti -Barmotin (Tight Juncion-Associated Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Tight junctions function to maintain cellular polarity and permeability barriers in epithelial and endothelial cells. Several molecules of the tight junction complex have been identified. The bartomin protein is preferentially localized at the periphery of tight junctions of hepatocytes and other epithelia. The antigen is also detectable in the intestine and kidney. Studies with Madin-Darby canine kidney (MDCK) cells indicate the antigen to be an important molecule for regulation of the barrier function of tight junctions. Confocal laser scanning microscopy revealed that the expression of the antigen decreased sequentially during hepatocarcinogenesis. The antigen was expressed intensely at the apical and basolateral membrane of cancer cells with glandular pattern. Examination of human gastric and colon cancer tissues showed that tight junctions were maintained highly in the well-differentiated (gland-forming) adenocarcinomas, but reduced in poorly-differentiated adenocarcinomas. In MDCK cells the disappearance of antigen expression is closely related with cell spreading in vitro induced hepatocyte growth factor/scatter factor.
Catalog #B0094-42
ApplicationsCan be used for Western Blot and Immunohistology on frozen sections.
Recommended DilutionWestern Blot: 1:10
Immunohistology: 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No6A484
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, containing 0.02% sodium azide and 0.2% BSA.
PurityPurified IgM
SpecificityReacts with a 155kD tight junction-associated protein also designated barmotin. Reacts with the human, canine, mouse, rat and chicken protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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