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You are here:Home » Antibodies » Abs to Cancer Markers, Leukemia » Anti -Bcl-2 (B Cell Leukemia 2) (FITC)

Anti -Bcl-2 (B Cell Leukemia 2) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells.
Catalog #B0807-06G
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50 (5ug/ml) labels 5x10e5 cells
Optimal dilutions to be determined by the researcher.
Flow cytometry Protocol1. Cultured Nalm-6 human tumor cells were fixed for 30 min with 2% buffered formaldehyde after which they were washed two times.
2. Subsequent reagent incubations and washes were done using a buffer containing 0.3% Saponin to permeabolize cells. 3. 5 x 10e5 cells per tube were incubated 45 minutes on ice with 80ul of B0807-06G at a dilution of 1:50 (5ug/ml).
4. Cells were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.09 log10 fluorescent units when compared to a mouse IgG1/FITC negative control at a similar concentration.
5. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-bcl-2 antibody (Catalog # B0807-06E).
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H161
HostMouse
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 5% glycerol, 0.2% BSA, 0.04% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman bcl-2 peptide aa41-54
SpecificityRecognizes the intracellular 26kD human bcl-2 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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