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B0807-06H Mouse Anti-Bcl-2 (B Cell Leukemia 2) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG1
Clone Number
7H161
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

The human bcl-2 gene family plays a central role in lymphoid system cell survival. Continuous expression of bcl-2 protein is critical for preventing apoptosis of mature peripheral B and T cells.

Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50 Western Blot: Unconjugated Optimal dilutions to be determined by the researcher..
Flow Cytometry Protocol
Incubations and washes using a buffer containing 0.5% Tween-20 to permeabolize cell, were done at RT. 1. Cultured Nalm-6 human tumor cells were fixed for 20 min with 0.5% freshly prepared buffered formaldehyde after which they were washed two times. 2. Five x 10e5 cells per tube were incubated 45 minutes with 80ul of B0807-06H at a 1:50 dilution.  3. Cells were then washed three times, fixed and analyzed using a BD FACstar plus. 4. Cells stained positive with a mean shift of 0.78 log 10 fluorescent units when compared to a tube where cells were pre blocked with 20ul of 0.5mg/ml B0807-06E.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of enzymatic activity
Immunogen
Human bcl-2 peptide 41-54.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the intracellular 26kD of the human Bcl-2 protein.
References
1. F. Pezzella, et al, (1990) Am J Pathol 137: 225-232. I.A. Doussis, et al, (1993) Am J Clin Pathol 99: 663-667. 2. S. Cory, (1995) Annu Rev Immunol 13: 513-543.
USBio References
No references available
Conjugates
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