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B0807-11Z Mouse Anti-Bcl-9 (B Cell CLL/Lymphoma 9, MGC131591)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
O00512
Isotype
IgG2b
Clone Number
9H67
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a 149kD transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta-catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulatory mechanisms. Human Bcl-9 is 1426aa in length. It contains one phosphothreonine and three phosphoserine sites, two poly-Pro regions (aa514-517 and 970-973), and one poly-Ala segment (aa900-903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391-1426. Over aa1009-1328, human Bcl-9 is 96% aa identical to mouse Bcl-9. B cell cancers often have translocations at the 3’UTR region of the Bcl-9 gene.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
E. coli-expressed recombinant protein corresponding to Met1009-Gly1328 of human Bcl-9, aa1009- 1328 (UniProt accession #O00512).
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes endogenous human Bcl-9 (Mr ~150kD).
Conjugates
Pricing
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