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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -Bcl-9 (B Cell CLL/Lymphoma 9, MGC131591)

Anti -Bcl-9 (B Cell CLL/Lymphoma 9, MGC131591)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a 149kD transcriptional regulator that
belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit
Pygopus to the Wnt-pathway beta-catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are
considered evolutionary duplicates of Legless that perform the same task with different regulatory mechanisms. Human Bcl-9 is 1426aa in length. It contains one phosphothreonine
and three phosphoserine sites, two poly-Pro regions (aa514-517 and 970-973), and one
poly-Ala segment (aa900-903). There is one potential alternate start site at Met27, and a variant
isoform exists that shows a four aa substitution for aa 1391-1426. Over aa1009-1328, human
Bcl-9 is 96% aa identical to mouse Bcl-9. B cell cancers often have translocations at the 3’UTR
region of the Bcl-9 gene.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #B0807-11Z
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H67
HostMouse
SourceHuman
Concentration~500ug/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant human Bcl-9, aa1009- 1328; O00512 (E. coli).
SpecificityRecognizes human Bcl-9 at~149kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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