| Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a 149kD transcriptional regulator that |
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| belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit |
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| Pygopus to the Wnt-pathway beta-catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are |
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| considered evolutionary duplicates of Legless that perform the same task with different regulatory mechanisms. Human Bcl-9 is 1426aa in length. It contains one phosphothreonine |
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| and three phosphoserine sites, two poly-Pro regions (aa514-517 and 970-973), and one |
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| poly-Ala segment (aa900-903). There is one potential alternate start site at Met27, and a variant |
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| isoform exists that shows a four aa substitution for aa 1391-1426. Over aa1009-1328, human |
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| Bcl-9 is 96% aa identical to mouse Bcl-9. B cell cancers often have translocations at the 3’UTR |
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| region of the Bcl-9 gene. |
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| Applications: |
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| Suitable for use in Western Blot. Other applications not tested. |
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| Catalog # | B0807-11Z |
| Recommended Dilution | Western Blot: 0.5ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 9H67 |
| Host | Mouse |
| Source | Human |
| Concentration | ~500ug/ml |
| Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant human Bcl-9, aa1009- 1328; O00512 (E. coli). |
| Specificity | Recognizes human Bcl-9 at~149kD. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
