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You are here:Home » Molecular Biology » MB-Protein Kinases » Blk, active, Recombinant, Human (B lymphoid kinase, B lymphoid tyrosine kinase)

Blk, active, Recombinant, Human (B lymphoid kinase, B lymphoid tyrosine kinase)

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Specifications

Blk, also known as B lymphoid kinase, is a 55 kd tyrosine kinases with a SH3, SH2 and catalytic domains that contain consensus sequences of the src protein tyrosine kinase family. Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells (1). Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk (2). Upon activation, the SH2 domain of Blk binds more than 10 distinct phosphoprotein including those of 72, 76, 115, and 130 kd (3). Blk prefers peptide substrates of the structure I/L-Y-D/E-X-L which resemble critical features of the ITAM motifs found in, e.g. the intracellular components Ig-alpha and Ig-beta of the beta cell receptor. Blk has a strong preference for a negatively charged amino acid in position +1 (4).
Catalog #B1900-01A
Source Recombinant full-length human BLK containing N-terminal GST tag expressed by baculovirus in Sf 9 insect cells.
Specific Activity 579nmol phosphate incorporated into Poly(Glu-Tyr) per minute/mg protein at 30ºC using a final concentration of 50uM ATP (0.83uCi/assay).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~84kD
SourceBaculovirus infected insect Sf9 cells
Purity90% (SDS-PAGE and Coomassie blue staining)
ConcentrationAs reported
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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