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B2125-26 Mouse Anti-Blood Group Kp(bc) (Kell antigen)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
BRIC 203
Grade
Affinity Purified
Applications
IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Fusion Partners: Spleen cells from immunized Balb/c were fused with cells of a myeloma cell line.

Recommended Negative Controls: Mouse IgG1 Negative Control
Applications
Immunoprecipitation Hemagglutination Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human erythrocytes.
Form
Supplied as a liquid in Tris buffered glycine, pH 8.0, with 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the kell blood group antigen Kpbc. The kell blood group antigens are carried on a 93kD erythrocyte membrane glycoprotein. In addition clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but does not react with Ko erythrocytes.
References
1. Parsons, S.F. et al. (1992). Monoclonal antibodies against kell glycoprotein: serology, immunochemistry and quantification of antigen sites. Transfusion Medicine 3: 137-142.
USBio References
No references available
Conjugates
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