Anti -Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IC |
|
| Bmi1 is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background, a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer. | | | Applications: | | | Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. | | | Catalog # | B2185 | | Recommended Dilutions | Western Blot: 0.2-2ug/ml. Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with B2185. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. | | Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. | | Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde. | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Control | U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 1.T.21 | | Host | Mouse | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-Glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant protein corresponding to aa1-202 of mouse Bmi-1 (NP_005171). | | Specificity | Recognizes mouse Bmi-1 (triplet), Mr 40-44kD.Species Crossreactivity: human, rat and rabbit. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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