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B2185 Mouse Anti-Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Swiss Prot
P35226, P25916
Isotype
IgG1
Clone Number
1.T.21 (F6)
Grade
Affinity Purified
Applications
IC IP WB
Crossreactivity
Hu Mo Rb Rt
Accession #
NP_005171
Shipping Temp
Blue Ice
Storage Temp
-20°C

Bmi1 is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background, a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.

Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.2-2ug/ml. Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with B2185. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde. Optimal dilutions to be determined by the researcher.
Recommended Positive Control
U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1-202 of mouse Bmi-1.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes mouse Bmi-1 (triplet), Mr 40-44kD. Species Crossreactivity: human, rat and rabbit
References
1. Voncken, J.W., et al., J. of Cell Science 112: 4627-4639 (1999). 2. Jacobs, J.J.L., et al., Genes Dev. 13: 2678-2690 (1999). 3. Jacobs, J.J.L., et al., Nature 397: 164-168 (1999). 4. Alkema, M.J., et al., Genes. Dev. 11: 226–240 (1997). 5. Itahana, K. et al., Mol Cell Biol. (2003) 23:389-401. 6. Lindstrom, M. S. et al., (2001) 20; 2171-2177. 7. Song, Li-Bing, et al. (2006). Cancer Res. 66: 6225-32.
USBio References
No references available
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