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You are here:Home » Antibodies » Antibodies-Transcription Factors, Zinc (Ring) Finger » Anti -Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)

Anti -Bmi-1, aa1-202 (RNF51, MGC12685, BUP, Polycomb Group RING Finger Protein 4)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Bmi1 is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background, a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested.
Catalog #B2185
Recommended DilutionsWestern Blot: 0.2-2ug/ml. Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with B2185. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts.
Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde.
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlU2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No1.T.21
FormSupplied as a liquid in 0.1M Tris-Glycine, 0.15M sodium chloride, pH 7.4, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa1-202 of mouse Bmi-1 (NP_005171).
SpecificityRecognizes mouse Bmi-1 (triplet), Mr 40-44kD.Species Crossreactivity: human, rat and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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