The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas (1). BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence (2). Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells (3), suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.

Suitable for use in Western Blot. Other applications not tested.

Western Blot: 0.5-1ug’ml Optimal dilutions to be determined by the researcher.

K562 cell lysate

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide BMI-1, 15 aa near the center of human BMI-1 (UniProt # P35226).

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

Purified by immunoaffinity chromatography.

Recognizes human BMI-1. Species Crossreactivity: mouse and rat.