Cyclic adenosine monophosphate or cAMP is an intracellular by-product of ATP by adenylene cyclase when G-protein Coupled Receptors (GPCR) bind various ligands on the cell surface. Cyclic AMP acts as a second messenger for signal transduction across the cell membrane and is a part of kinase activation. It regulates the effects of glucagon and adrenaline along with the movement of calcium ions through ion channels. Since it is affected directly by GPCR activation, cAMP is studied as a means to monitor GPCR in the discovery of therapeutic drugs.
Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Paraffin): 1:100. Requires HIER using sodium citrate buffer, pH 6.0. Optimal dilutions to be determined by the researcher.
Positive Control
Rat hippocampus and cerebellum tissue
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
2'O-succinyl cyclic AMP conjugated to BSA.
Form
Supplied as a liquid in PBS. No preservative added.
Specificity
Recognizes cAMP. Has been shown to react with rat. Expected to recognize cAMP from all species. Specific for cAMP with no cross reaction to other nucleotides at concentrations to 1mM. Reactive with cAMP fixed in tissues or acetylated cAMP. Does not react with free, non-acetylated cAMP in RIA or EIA assays.