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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Bradford Protein Assay Kit

Bradford Protein Assay Kit

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Specifications

The protein is known as the "building blocks of life" and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. Protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides.
Catalog #B2674-95
ApplicationsDirect Assays: Total protein concentration
Key FeaturesSensitive and accurate: Use 10ul samples. Detection range 0.06-1.0mg/ml protein in 96-well plate assay.
Simple and high-throughput: The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Low interference: Glucose, Tris, vitamins and amino acids, DNA, RNA, salts, EDTA (<12mM), phenol (<50mM), urea (<0.6M), Triton (<0.1%) and SDS (<0.1% do not interfere in the assay.
Versatility: Assays can be executed in 96-well plate or cuvet.
Kit ComponentsB2674-95A: Reagent (5x), 1x20ml. Supplied as a liquid in 48% Phosphoric acid, 25% Ethanol, <0.05% Brilliant Blue G, <0.05% Triton X-100
B2674-95B: Protein Standard, 1x1ml. Supplied as a liquid, 1mg/ml BSA
Storage and StabilityStore B2674-5A at 4°C in the dark. Store B2674-95B at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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