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B2702-01N Goat Anti-Brain Natriuretic Peptide Pro (proBNP, Natriuretic Peptides B, Gamma-brain Natriuretic Peptide, NPPB)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Human
Swiss Prot
P16860
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human BNP is a 3.5kD, secreted, nonglycosylated peptide that belongs to the natriuretic peptide family. It is the product of ventricular myocytes and bone marrow endothelial cells. BNP is synthesized as a 12kD, 108aa prohormone with a 76aa prosegment and a 32aa mature (BNP) region. Both forms circulate, with the prohormone showing O-linked glycosylation that generates a 25-27kD glycoprotein. Glycosylation is suggested to stabilize the molecule. The proform also tends to noncovalently trimerize. In blood, 1-32 BNP is proteolytically processed into 3-32 BNP, with loss of Ser-Pro at the N-terminus. Human Pro-BNP is 29% and 50% aa identical to mouse and pig Pro-BNP.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to His27-His134 from human Pro-BNP expressed in E.coli.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Pro-BNP.
Conjugates
Pricing
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