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You are here:Home » Antibodies » Antibodies-Hormones, Steroids » Anti -Brain Natriuretic Peptide Pro (proBNP, Natriuretic Peptides B, Gamma-brain Natriuretic Peptide, NPPB)

Anti -Brain Natriuretic Peptide Pro (proBNP, Natriuretic Peptides B, Gamma-brain
Natriuretic Peptide, NPPB)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Human BNP is a 3.5kD, secreted, nonglycosylated peptide that belongs to the natriuretic peptide family. It is the product of ventricular myocytes and bone marrow endothelial cells. BNP is synthesized as a 12kD, 108aa prohormone with a 76aa prosegment and a 32aa mature (BNP) region. Both forms circulate, with the prohormone showing O-linked glycosylation that generates a 25-27kD glycoprotein. Glycosylation is suggested to stabilize the molecule. The proform also tends to noncovalently trimerize. In blood, 1-32 BNP is proteolytically processed into 3-32 BNP, with loss of Ser-Pro at the N-terminus. Human Pro-BNP is 29% and 50% aa identical to mouse and pig Pro-BNP.
Catalog #B2702-01N
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa27-134 from human Pro-BNP expressed in E.coli.
SpecificityRecognizes human Pro-BNP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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