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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Breast Cancer Resistance Protein (BCRP)

Anti -Breast Cancer Resistance Protein (BCRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IC
Tumor cells may display a multidrug resistant phenotype by overexpression of ATP-binding cassette transporters such as multidrug resistance (MDRI) p-glycoprotein, multidrug resistance protein 1 (MRP1), and breast cancer resistance protein (BCRP). Tumor cells can be intrinsically resistant to drugs or they can acquire resistance to structurally and functionally unrelated drugs on drug exposure. This phenomenon is known as multidrug resistance (MDR). In human tumor cells, several transporter proteins can be involved in MDR. These proteins, MDR1 P-gp (ABCB1), MRP1 (ABCC1), MRP2 (ABCC2), MRP3 (ABCC3), and BCRP (ABCG2), belong to the ABC transporter family. They act as efflux pumps, which result in decreased intracellular concentrations of cytotoxic drugs. BCRP is a recently discovered half-transporter that probably acts as a homo- or heterodimer in transporting cytotoxic agents. The transporter molecule is capable of transporting several anticancer drugs but has thus far been found mainly in MX-resistant cell lines.
Catalog #B2738
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 1:20-1:50 (acetone-fixed frozen cells).
Immunohistochemistry (FFPE): 1:20-1:40 following pretreatment in 10mM citrate buffer, pH 6.0,
for 20 min.
Immunohistochemistry (frozen): 1:20-1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No4i319
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS with 0.7% BSA and sodium azide.
PuritySupernatant
ImmunogenFusion protein composed of E. Coli maltose binding protein and BCRP peptide (126 amino acids, 271-396 of BCRP). Cellular Localization: plasma membrane, integral membrane protein.
SpecificityRecognizes an internal epitope of human BCRP at ~70kD. Does not react with the human MDR1, MRP1 and MRP2 gene products.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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