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B2739-70B Mouse Anti-Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
Q96GW7
Isotype
IgG1
Clone Number
8H196
Grade
Affinity Purified
Applications
E IP WB
Crossreactivity
Hu
Accession #
AAH09117
Shipping Temp
Blue Ice
Storage Temp
-20°C

Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa52-139) and a C-terminal EGF-like (aa646-682) and C-type lectin domain (aa695-802). Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa649-911 that serves as a GPI-anchor motif. Over aa23-911, human Brevican shares 82% aa identity with mouse Brevican.

Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Inhibition. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml under non-reducing conditions only. Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Brevican from conditioned cull culture medium. Inhibition (Blocking of receptor-ligand interaction): ~0.01-0.05ug/ml will block 50% of the binding of 1ug/ml of biotinylated Hyaluronan (132kD) to immobilized recombinant human Brevican (100ul of a 3ug/ml solution was coated in each well) in a functional ELISA. 1ug/ml will achieve >90% blocking in the assay. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Asp23-Pro911 from human Brevican expressed in NS0 cells
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant. Endotoxin <0.1EU/ug (LAL).
Specificity
Recognizes human Brevican. Also reacts with a truncated version (aa23-649) of human Brevican.
Conjugates
Pricing
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