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Anti -Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)
Anti -Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B IP |
|
| Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa 52-139) and a C-terminal EGF-like (aa 646-682) and C-type lectin domain (aa 695-802). Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa 649-911 that serves as a GPI-anchor motif. Over aa 23-911, human Brevican shares 82% aa identity with mouse Brevican. | | | Catalog # | B2739-70B | | Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Inibition. Other applications not tested. | | Recommended Dilutions | Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Brevican is ~1ng/well. | | Western Blot: 1-2ug/ml. The detection limit for recombinant human Brevican is ~10ng/lane under non-reducing conditions. | | Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Brevican from conditioned cull culture medium. | | Inhibition (Blocking of receptor-ligand interaction): ~0.01-0.05ug/ml will block 50% of the binding of 1ug/ml of Hyaluronan (132kD) to immobilized recombinant human Brevican (100ul of a 3ug/ml solution was coated in each well) in a functional ELISA. 1ug/ml will achieve 100% blocking in the assay. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 8H196 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein G affinity chromatography. Endotoxin 0.1EU. | | Immunogen | Recombinant protein corresponding to aa23-911 from human Brevican expressed in NSO cells (Q96GW7). | | Specificity | Recognizes human Brevican. Also reacts with a turncated version (aa23-649) of human Brevican. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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