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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Anti -Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IP
Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa 52-139) and a C-terminal EGF-like (aa 646-682) and C-type lectin domain (aa 695-802). Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa 649-911 that serves as a GPI-anchor motif. Over aa 23-911, human Brevican shares 82% aa identity with mouse Brevican.
Catalog #B2739-70B
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Inibition. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Brevican is ~1ng/well.
Western Blot: 1-2ug/ml. The detection limit for recombinant human Brevican is ~10ng/lane under non-reducing conditions.
Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Brevican from conditioned cull culture medium.
Inhibition (Blocking of receptor-ligand interaction): ~0.01-0.05ug/ml will block 50% of the binding of 1ug/ml of Hyaluronan (132kD) to immobilized recombinant human Brevican (100ul of a 3ug/ml solution was coated in each well) in a functional ELISA. 1ug/ml will achieve 100% blocking in the assay.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No8H196
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography. Endotoxin 0.1EU.
ImmunogenRecombinant protein corresponding to aa23-911 from human Brevican expressed in NSO cells (Q96GW7).
SpecificityRecognizes human Brevican. Also reacts with a turncated version (aa23-649) of human Brevican.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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