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Specifications

Clone Host Grade Applications Monoclonal Mouse IC FC IF

Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues (1,2). BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle (3,4). After pulse-labeling cells or tissues with bromodeoxy-uridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure as well as denaturation of double stranded DNA for various immunodetection applications.
Catalog #	B2850-10B
Applications	Suitable for use in Immunohistochemistry (P), Immunofluorescence (IF-IC) and Flow Cytometry
	Other applications not tested.
Recommended Dilution	Immunohistochemistry (P): 1:100 Unmasking buffer: Citrate
	Immunofluorescence (IF-IC): 1:1400
	Flow Cytometry: 1:800
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10E10
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and 0.02% sodium azide.
Immunogen	BrdU conjugated to BSA.
Specificity	Recognizes human BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.