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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » BseNI (BsrI)

BseNI (BsrI)

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Specifications

Catalog #B2915
Description5'-A C T G G N^-3'
3'-T G A C^C N-5'
SourceBacillus species N
Concentration 10u/ul
Unit DefinitionOne unit is defined as the amount of BseNI required to digest 1ug of lambda DNA in 1 hour at 65°C in 50ul of assay buffer.
Diluent BufferFor short term storage (3-4 weeks)- dilute with 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage BufferFor long term storage: 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Supplied WithR1625-01: Restriction Enzyme Buffer B, 10X
Suplied as a liquid in 10mM Tris-HCl pH 7.5, 10mM magnesium chloride, 0.1mg/ml BSA.
R1625 Restriction Enzyme Buffer A, 10X:
Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Enzyme Properties
Methylation Effects
DamNever overlaps - no effect
DcmNever overlaps - no effect
CpGNever overlaps - no effect
EcoKlMay overlap - effect not determined
EcoBlMay overlap - effect not determined
Stability during Prolonged Incubation
A minimum of 0.1units of BseNI is required for complete digestion of 1ug of DNA in 16 hours at 65°C.
Thermal Inactivation
BseNI is inactivated by incubation at 80°C for 20min.
Number of Recognition Sites in DNA
Lambda110
M13mp18/1918
pBR32219
pUC18/19, pUC5711
pTZ19R/U12
X1749
Quality Control
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BseNI.
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with BseNI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 1.0uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of BseNI for 4 hours.


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