Sequence
5'-T C G^C G A-3' 3'-A G C^G C T-5'
Source
Bacillus megaterium RFL68
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Diluent Buffer
10mM Tris-HCl pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer
10mM Tris-HCl pH 7.5, 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% glycerol.
R1625 Restriction Enzyme Buffer A, 10X
1X buffer formulation - 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.
R1625-03 Restriction Enzyme Buffer D, 10X
1X buffer formulation - 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM sodium chloride and 0.1mg/ml BSA.
Labeled Oligonucleotide (LO) Assay
Dam
May overlap - no effect
Dcm, EcoKI, EcoBI
Never overlaps - no effect
CpG
Completely overlaps - blocked
Stability during Prolonged Incubation
Digestion of Agarose-embedded DNA
Number of Recognition Sites in DNA