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Specifications

C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR). Murine C2C12 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1mM sodium ethylenediaminetetraacetate, 1mM phenylmethylsulfonyl flouride, 1 ug/ml of aprotinin, 5ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1X SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Catalog #	C0009
Note	Cell line-C2C12 (muscle; myoblast); Growth media-DMEM & 10% fetal bovine serum.
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 20ug per lane, mini gel.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Source	Murine muscle - C2C12 (Murine myoblast cell line) Whole cell lysate
Concentration	~1mg/ml
Form	Supplied as a whole lysate in 1X SDS, 5% b-mercaptoethanol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.