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Anti -Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)
Anti -Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1,
D22S11)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IC FC |
|
| The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The N-terminal part of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domain. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The C-terminal part may be involved in a GTPase-activating function for the small GTP-binding protein Rac (2,3). The function of Wildtype Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstrean signal mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyrosine 177 of Bcr is phosphorylated in Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl | | | (6). Phosphorylated tyrosine 177 of Bcr provides a docking site for Gab2 and GRB2 (7,8). | | | Catalog # | C0013-01C | | Applications | Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 | | Immunocytochemistry: 1:100 | | Flow Cytometry: 1:25 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. | | Purity | Purified by protein A and immunoaffinity chromatography. | | Immunogen | Synthetic peptide (KLH coupled) corresponding to the N-terminal sequence of human Bcr. MW of Antigen: 130, 160kD | | Specificity | Detects endogenous levels of total Bcr and the 210kD Bcr-Abl fusion protein. Species Crossreactivity: Human, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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