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Anti -c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free)
Anti -c-erbB2, p185 (HER2, neu Receptor) (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP IH |
|
| c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer. Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy. | | | Catalog # | C0015X | | Applications | Suitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilutions | Immunoprecipitation: 10ug/mg protein lysate. Use Protein A. | | Western Blot: 5-10ug/ml for 2 hours at RT | | Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. | | Optimal dilutions to be determined by the researcher. | | Positive Control | SKBR-3 cells, T47D cells or breast carcinomas. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4. No stablilizing proteins and no preservatives added. Available with BSA and sodium azide. See C0015. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide derived from the C-terminus of human c-erbB-2/HER-2 protein. Sequence is identical in rat neu protein. Cellular Localization | | Specificity | Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Species Crossreactivity: monkey, rat and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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