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C0026-09D Rabbit Anti-ErbB3, phosphorylated (Tyr1222) (c-erbB3, HER3)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
P21860
Isotype
IgG
Clone Number
7H10(50C2)
Grade
Purified
Applications
E WB
Crossreactivity
Hu Mo
Gene ID
2065
Shipping Temp
Blue Ice
Storage Temp
-20°C

HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase. There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2 and the p85 subunit of PI3 kinase, which mediate ErbB- downstream signaling. Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3 kinase. ErbB3 is highly expressed in many cancer cells and activation of ErbB3-PI3 kinase pathway is correlated with malignant phenotypes of adenocarcinomas. In tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members, e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation. Thus, prevention of the interaction of ErbB3 with other ErbB tyrosine kinases has become a novel anti-tumor strategy.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr1222 of human ErbB3.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous human ErbB3 only when phosphorylated at tyrosine 1222. Does not crossreact with other tyrosine phosphorylated receptor tyrosine kinases. Species Crossreactivity: mouse
References
1. Yarden, Y. and Sliwkowski, M.X. Nature Rev. Mol. Cell. Biol. 2: 127–137 (2001). 2. Guy, P.M. et al. Proc. Natl. Acad. Sci. USA 91; 8132–8136 (1994). 3. Songyang, Z. et al. Cell 72: 767–778 (1993).4. Kim, H.H. et al. J. Biol. Chem. 269: 24747–24755 (1994) . 5. Sithanandam, G. et al. Carcinogenesis 24:1581–1592 (2003). 6. Kobayashi, M. et al. Oncogene 22: 1294–1301 (2003). 7. Holbro, T. et al. Proc. Natl. Acad. Sci. USA 100: 8933–8938 (2003).
USBio References
No references available
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