| Function: SwissProt: P54284. The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting. |
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| Size: 484 amino acids; 54532D |
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| Subunit: The L-type calcium channel is composed of four subunits: alpha-1, alpha-2, beta and gamma. |
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| Entrez Gene Number: NM_000725.2 |
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| Catalog # | C0104-80 |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:200-1:300 using ECL on rat brain membranes. Dilutions should be made using a carrier protein such as BSA (1-3%) |
| Immunohistochemistry: rat hippocampus and mouse cerebellum frozen sections. |
| Optimal dilutions to be determined by the researcher. |
| Control Antigen | C0104-80A |
| Preadsorption Control | 1ug peptide per 1ug antibody. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~0.3mg/ml |
| Form | Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 5% sucrose, 0.025% sodium azide. Reconstitute with 50ul sterile dH2O. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa463-477 [(C)DRNW QRNRP WPKDS Y] of rabbit Ca-beta 3. Epitope location: Intracellular, C-terminus. |
| Specificity | Recognizes rat Ca-beta 3. Recognizes the full length Ca beta3 protein, not with other known proteins tested so far. Species Sequence Homology |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
